Bioorthogonal chemical tagging of protein cholesterylation in living cells.
نویسندگان
چکیده
We report the first chemical probe for bioorthogonal chemical tagging of post-translationally cholesterylated proteins with an azide in living cells. This enables rapid multiplexed fluorescence detection and affinity labelling of protein cholesterylation, as exemplified by Sonic hedgehog protein, opening up new approaches for the de novo identification of cholesterylated proteins.
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ورودعنوان ژورنال:
- Chemical communications
دوره 47 14 شماره
صفحات -
تاریخ انتشار 2011